Scientist, Purification Protein Sciences Early Stage CMC Process Development - San Diego

Recruiter
Synthorx, Inc.
Location
San Diego, California
Posted
Nov 08, 2019
Closes
Nov 21, 2019
Ref
171e22d2274d
Sector
Science
Organization Type
Corporate
Scientist, purification protein sciences and early stage CMC process development Synthorx is a pharmaceutical company using a novel synthetic biology platform for the discovery and production of improved protein therapeutics. We have advanced a revolutionary technology that expands the genetic alphabet to drive the site-specific incorporation of multiple non-natural amino acids into proteins of any size, and to manufacture these improved proteins at scale. This technology enables the design of biotherapeutics with useful chemical functionality and opens-up opportunities to make novel protein therapeutics to previously difficult to drug targets. We are hiring a Scientist to join the Protein Sciences and CMC group. The successful candidate will have a strong background in protein biochemistry and purification and a working knowledge of protein expression in E. coli.

Key Responsibilities:
• Leadership role in protein production to support Late Stage Research and CMC activities.
• Strong subject matter expertise in downstream purification operations, including chromatography (affinity, ion exchange, HIC, reversed phase and multi-mode), membrane separation, targeted precipitation, protein refolding of inclusion bodies, etc.
• Experimentally determine methods to optimize recovery yields from protein expression.
• Experience with protein purification equipment at bench, pilot and clinical scale.
• Experience in authoring experimental protocols and reports, technology transfer process descriptions.
• Prepare and present project updates to colleagues, senior leadership, and external partners.
• Supervise/Mentor junior staff and provide insight and technical expertise for project progression.
• Support the team in the set-up, operation, and associated analysis of upstream and downstream processes.
• Maintain a high standard for quality control including up-to-date data records for all protein deliveries, including basic understanding of analytical methods for product quality and process decision making.
• Troubleshoot challenging technical, experimental, and instrument issues.
• Consummate ability to work in a highly collaborative, fast-paced, cross-functional environment, with superior oral and written communication skills.

Required:
• Ph.D. degree with 3+ or MSc degree with 10+ years of hands-on experience with recombinant protein production.
• Technical proficiency in designing and developing purification processes for proteins including products expressed in microbial hosts.
• Proficient in downstream purification using the Unicorn system with AKTA instruments.
• Familiarity with protein analytical techniques to perform assays such as HPLC, SDS-PAGE and endotoxin quantification.
• Experience in TFF (ultra-filtration, diafiltration) depth filtration and chromatography.
• Experience with E. coli-based protein expression.
• Effective communication and rapid prioritization to match dynamic and demanding timelines.
• Proven track record of success in anticipating and tackling non-standard challenging issues in upstream and downstream process development.
• Ability to work efficiently to meet aggressive deadlines and solid multitasking skills.

Travel:

0-20%

Additional preferred skills:
• Familiarity with scale-up preparation employing fermentation instrumentation and protocols.
• Experience in GMP and regulatory submissions.
• Basic understanding of cancer and/or immuno-biology.

Similar jobs

More searches like this

Similar jobs