Microbiology Technician

Expiring today

Employer
Pfizer
Location
Cambridge, Massachusetts
Salary
Competitive
Posted
Apr 28, 2021
Closes
May 07, 2021
Ref
544810
Jobseeker Type
Professional
Organization Type
Corporate
Hours
Full Time
ROLE SUMMARY

Pfizer's BioMedicine Design (BMD) department in Cambridge, MA is responsible for discovering and developing biotherapeutic drug candidates. The High Throughput (HTP) Protein Expression and Purification Group in BMD is an integral part of the early discovery

process and is responsible for producing thousands of transiently-expressed and purified antibodies and antibody-like proteins across the early portfolio of projects.

The HTP group is currently looking for a laboratory technician to provide DNA production and cell culture support for the protein expression and purification activities in BMD. Using automated liquid handling equipment, the successful candidate will grow and maintain small-scale bacterial cultures and purify and normalize DNA for mammalian cell transfections, and maintain a cataloged inventory of routinely used DNA. Additionally, the candidate will grow and maintain mammalian cells using aseptic technique. The candidate will join a team of scientists responsible for transient protein expression and purification of hundreds of proteins each week.

ROLE RESPONSIBILITIES

• DNA production/ purification/ characterization/inventory cataloging

• Mammalian cell culture

• Sample management and documentation

• Request database use and management

• Use of automated instrumentation

• Requestor interface

The successful candidate will be responsible for small scale DNA production activities, including automated DNA purification, quantitation, and normalization. The candidate will also help in passaging and maintaining mammalian cells required for transient transfections. The candidate will help manage DNA inventories and will be expected to assist with protein production request, which includes interfacing with other scientists in the department.

The successful candidate will have the ability to maintain an organized and clean laboratory space, maintain a rotating stock of supplies, communicate clearly on daily work, compile results in an organized lab notebook/online database, and consistently perform specific protocols. The candidate must be able to work closely with a team and communicate effectively to meet schedules and deadlines.

Education:
  • B.Sc in biological/life sciences or Associates/ Certificate in Biotechnology, or High School diploma with at least 2 years of relevant laboratory research experience


Qualifications
  • Previous microbiology experience
  • Previous experience working with DNA purification
  • Previous mammalian cell culture experience preferred
  • Must have good practical skills in a laboratory setting such as keeping an organized laboratory notebook and maintaining laboratory cleanliness to minimize contamination
  • Must be able to multitask
  • Must be able to follow detailed protocols
  • Must have ability to concentrate while doing repetitive tasks
  • Must be able to read thoroughly and understand weekly schedules


Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

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