Postdoctoral Fellow - Protein Engineering

The Williams lab is looking for a Postdoctoral Fellow interested in seeing their efforts translated to the clinic to treat cancer and diabetic patients. The position involves an atoms-up approach to design novel biologics to mitigate on-target, off-tissue toxicities and improve their therapeutic efficacy. The requisite research includes the application of structural biology, computation, biophysics, in vitro and in vivo assays while communicating and coordinating these efforts with the rest of the team and clinicians at City of Hope focusing on immediate clinical needs. The successful candidate will be deeply involved through all aspects of this bench-to-bedside approach.

The Williams lab is equipment with state-of-the-art instruments including multiple incubators, biosafety cabinets, multiple FPLCs (including a new Biorad NGC FPLC - automating the purification of dozens of molecules per day), an Agilent HPLC, Biacore T200, Waters nanoITC, Beckman XLI, Mosquito crystallization robot, Formulatrix imagers, and a Rigaku HF diffractometer. A new Sony SH800 cell sorter is also located in the lab to further drive these efforts using novel display libraries developed in-house. In addition, the successful candidate will have access to 27 core facilities across the campus.

Basic education, experience and skills required for consideration:

PhD in chemistry, biochemistry, protein chemistry, biochemical engineering or related field with 1 to 3 years of postdoctoral experience
Experience with protein expression and purification
Fully versed in protein purification methods (Affinity, SEC, IEX), analysis (e.g., SDS-PAGE)
Experience with cell culture (E. coli and CHO a must, yeast and insect desirable). Experience with SPR and other biophysical methods desirable
Protein engineering, production, purification in CHO
Biophysical characterization (SPR, ITC, AUC, CD)
X-ray Crystallography
Assay development, interpretation, and troubleshooting
Excellent written and verbal communication skills
Good problem-solving skills
Ability to conduct independent research
Ability to supervise others and work effectively in a team

Preferred education, experience and skills:

Enzymology experience a plus
CryoEM experience a plus