Scientist, Gene Editing Technology (Multiple Openings)

OPPORTUNITY
Mammoth is seeking a Scientist with expertise in building genome editing tools in mammalian contexts to join our growing genome editing technology team. The ideal candidate will have direct experience with developing novel CRISPR-based genome manipulation technologies including, but not limited to, gene editing, gene regulation, epigenetic modulation, and base editing. A strong background in mammalian cell engineering and synthetic biology is essential. Candidates must be comfortable collaborating with others and prioritizing time, agendas, and projects to accomplish goals.
KEY RESPONSIBILITIES

• Screen CRISPR Cas systems to test their potential use in complex genome manipulation tools
• Construct and validate various CRISPR based genome manipulation tools in mammalian cells
• Design CRISPR-based genome manipulation tools with novel modalities
• Build assays to evaluate diverse genome manipulation outcomes including on-target and off-target effects
• Keep up with relevant literature on the latest developments in the field
• Communicate key scientific data to project teams and senior management with a clear interpretation of data and proposal of next steps
• Collaborate with others on the team and, when applicable, manage direct reports

REQUIRED QUALIFICATIONS

• Ph.D. in molecular and cell biology, bioengineering, biochemistry, or a related field
• 3+ years of experience in (an) academic and/or industry setting(s)
• Hands-on experience of CRISPR based gene editing machinery development and assessment in mammalian cells
• Experience using one or more gene-editing tools (e.g gene regulation, epigenetic modulation, and/or base editing) with an understanding of their optimization in mammalian cells
• Demonstrated experience with mammalian cell culture of different mammalian cell types, both primary and immortalized cells
• Experience with DNA/RNA isolation, molecular cloning, and PCR/qPCR/next-generation sequencing experiment design and execution
• Proficiency in cell biology skills such as flow cytometry and FACS

PREFERRED QUALIFICATIONS

• Experience with mRNA and protein production of gene-editing components
• Familiarity with various mammalian cell delivery strategies and their optimization process, including lipid-based transfection, electroporation, viral vector, etc.
• Experience with luminescence/fluorescence cellular assays
• Proficient in R/Matlab/Python or other programming languages and familiarity with their usage in bioinformatics data analysis.
• Experience with protein engineering to improve gene editing tools

BENEFITS

• Company-paid health/vision/dental benefits
• Unlimited vacation and generous sick time
• Company-sponsored meals and snacks
• Wellness, caregiver, and ergonomics benefits
• 401(k) with company matching

Mammoth Biosciences is harnessing the diversity of life to power the next generation of CRISPR products. Through the discovery and engineering of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. Mammoth aims to develop permanent genetic cures through best-in-class in vivo and ex vivo therapies and to democratize disease detection with on-demand diagnostics. Mammoth can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Based in the San Francisco Bay Area, Mammoth Biosciences is co-founded by CRISPR pioneer and Nobel Laureate Jennifer Doudna and Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Redmile Group, Foresite Capital, Senator Investment Group, Sixth Street, Decheng, Mayfield, NFX, and 8VC, along with leading individual investors including Brook Byers, Tim Cook, and Jeff Huber.

TEAM
Our small-size proteins, such as Cas14 and CasPhi, are poised to transform disease treatment enabling novel editing approaches and delivery options that are difficult to achieve or not even possible with previously described CRISPR-Cas systems. Our teams are responsible for discovering and engineering novel CRISPR systems, establishing CRISPR-based diagnostics, and translating our unique CRISPR technology into therapeutic treatments with the ultimate goal of cures for indications with high medical need.

It is our policy and intent to provide equal opportunity to all persons without regard to race, color, religion, political affiliation, sex/gender (including gender expression/identity, pregnancy, childbirth and related medical conditions), marital status, registered domestic partner status, sexual orientation, age, ancestry, national origin, veteran status, disability, medical condition, genetic characteristics, and/or any other basis protected by law. This policy covers all facets of employment including, but not limited to: recruitment, selection, placement, promotions, transfers, demotions, terminations, training, and compensation.