Research Associate - Marson Lab

Category:Science
Lab/Area:Marson LabDescription:
The Marson Lab is recruiting a Research Associate to assist with ongoing projects to understand gene regulatory networks in primary immune cells. Our lab has pioneered the application of novel genetic engineering technologies such as CRISPR to primary human immune cells (Schumann et al., PNAS 2015). These new technical abilities have furthered our labs research into the genetic underpinnings of autoimmune disease and T cell biology (Farh and Marson et al., Nature 2015; Calderon et al., Nature Genetics 2019), as well as opened the door to novel cancer immunotherapies based on engineered human immune cells (Roth et al., Nature 2018). In addition to cell editing, our lab integrates genomic analysis (e.g. ChIP-seq, ATAC-seq, RNA-seq), human disease genetics, and high dimensional screening approaches for in vitro and in vivo studies of T cell function (Simeonov et al. Nature 2017; Shifrut and Carnevale et al. Cell 2018; Freimer and Shaked et al. BioRxiv 2021).

Working as part of a multidisciplinary group, the Research Associate will perform pooled and arrayed screening experiments to map gene regulatory networks in primary immune cells. Primary responsibilities will include isolating and culturing of primary human immune cells, genetic editing of immune cells using CRISPR/Cas9, producing lentivirus, performing flow cytometry and fluorescence activated cell sorting, and producing next generation sequencing libraries. Responsibilities will also involve basic molecular biology (PCR, cloning, preparing plasmid constructs, etc.). Strong organizational and communication skills are required. This position represents an excellent opportunity to gain exposure to basic laboratory skills, genomics, and genome engineering in a dynamic, well-established research environment.

Required Qualifications:

• BA/BS in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; or an equivalent combination of education and experience

• Experience with general laboratory techniques, especially basic molecular and cellular biology techniques
• Willingness and ability to learn new methods and skills for changing research priorities
• Ability to work independently and as a member of a research team
• Strong interpersonal communication skills (verbal and written)
• Strong organizational skills
• Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines

Preferred Qualifications:

• One or more years of laboratory experience utilizing techniques or methods required by the position
• Prior experience with cell culture, especially T cell culture
• Prior experience with next-generation sequencing library preparations and quality control
• Prior experience in writing reports to include methods/protocols/procedures, results and tentative conclusions

Payment is according to relevant experience. The Gladstone-UCSF Institute of Genomic Immunology is an equal opportunity/affirmative action employer. All qualified candidates are encouraged to apply.

Additional Information:

• Title and salary will be commensurate with education and experience.
• Be a part of big ideas and consider Gladstone. Your difference will make a difference in human health.
• Gladstone is an equal opportunity employer.