Working under moderate supervision, the Research Assistant 1 will perform research work in the co-expression and purification of NBCe1-B and IRBIT, as well as the NBCe1-A. NBCe1-B and NBCe1-A, play important roles in acid secretion by the kidney; HC03, in secretion by the pancreas and in cell-pH regulation throughout the body
* Perform research work in medical and related technical areas involves use of lab skills. Express proteins in HEK293 cells in suspension, maintain cell cultures on a daily basis, and split cultures weekly. Transduce cells in suspension. (40%)
* Conduct analysis of samples and data interpretation of expressed proteins. (15%)
* Record results for a particular experiment or closely related series of experiments, including documentation of process for expression of protein. (10%)
* Maintain records of experiments, keeping detailed records of experiments in either lab work or electronic record. (10%)
* Maintain test equipment utilized in experiments. Clean labware. (10%)
* Order laboratory supplies. Order reagents and make culture solutions (10%)
Perform other duties as assigned. (5%)
Department: Daily contact with supervisor to discuss research and maintain workflow.
University: Occasional contact with other departments to share information.
External: Limited or no contact with vendors to exchange information.
Students: Contact with student employees to exchange information.
This position has no direct supervision of staff employees.
Education/Experience: Bachelor's degree in science and 0 to 1 year of experience or Associate's degree in an approved biotechnology program and 0 to 2 years of experience required.
* Knowledge of commonly used concepts, practices and procedures in chemistry and biology.
* Ability to follow instructions and pre-established guidelines to perform the functions of the job.
* Effective communication skills.
* Excellent problem-solving skills.
* Good interpersonal skills: ability to work and communicate with individuals within the laboratory.
* Strong organization skills: ability to multi-task, prioritize and give attention to detail in order to complete tasks.
* Ability to meet consistent attendance requirements.
* Ability to work in a changing environment and conform to shifting priorities, demands and timelines.
* Ability to adapt to varying workloads and respond appropriately to stressful situations.
* Ability to complete all training requirements as set by the university and appropriate regulatory agencies.
* Ability to learn how to operate standard laboratory equipment.
* Ability to interact with colleagues, supervisors and customers face to face.
General Laboratory environment. Work performed primarily in a lab setting with frequent interruptions and interactions. The employee is regularly exposed to research scientific chemicals and not exposed to explosive hazards, burn hazards and electrical shock. May be required to use personal protective equipment, including standard lab coat, gloves and safety glasses when appropriate. Noise level is moderate.