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UNIV - PostDoc - Pediatrics: DCRI

Employer
MUSC Health University Hospital
Location
Charleston County, SC
Closing date
Jan 15, 2022

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Sector
Doctors, Pediatrics
Organization Type
Corporate
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Job no: 539800
Entity: MUSC University
Department: COLLEGE OF MEDICINE - COM-Pediatrics
Sub Department: UNIV-2248960-Childrens Research Institute
Work type: Full-Time
Employee Type: UNIV - Post Doc
Location: Charleston, SC-Downtown
Categories: Research
Pay scale: UNIV-Band 0: Commensurate with experience.

FLSA: Salaried

Job Summary: A cancer research laboratory in the Hollings Cancer Center and Darby Childrens Research Institute has a job opening for a postdoctoral position. The lab uses in vitro, ex vivo and in vivo models to elucidate the intracellular drivers of pediatric brain tumor growth. The research will particularly focus on using genetic mouse models and patient derived orthotopic xenografts to dissect the drivers of medulloblastoma tumor growth and relapse. Studies will use a range of complementary methods including cellular and molecular biology (immunoblotting, RT-qPCR, immuno-precipitation), IHC and IF imaging, flow cytometry methods, single-cell RNAseq, and functional cell-based assays. The candidate should be highly motivated with a recent Ph.D. degree and a solid background and skills in molecular/cell biology and biochemistry.

Candidate should send CV directly to Dr. Jezabel R Blanco by email (rblanco@musc.edu).

Payscale Salary Range: UNIV-Band 0: Commensurate with experience.

Job Duties:

  • 30% - Collaborates with the PI and other lab members involved to design and executes experiments using state-of-the-art mouse genetic models and cells prepared from these models to identify drivers of medulloblastoma growth and relapse. These duties included genotyping of genetically-engineered mice and animal husbandry, in vivo experiments, including treatment studies and orthotopic/subcutaneous tumor cell implantation, monitoring for development of disease/tumor growth, necropsies of experimental mice and in vitro studies, including preparing cells from the models, cell culture, molecular (cell viability, IF, FACs analyses, immunoblotting, PCR, qPCR, reporter assays, IHC, ChIP), and functional genomic evaluation of gene expression and transcription factor binding to target genes, including single cell RNAseq, standard RNAseq and ChIPseq. Bacterial recombinant DNA work and lentiviral production for in vitro transfection and transduction experiments. - (Essential)
  • 30% - Collaborates in collecting and analyzing data generated from the experiments. Duties include keeping accurate records on a daily basis, identifying and using appropriate software for analysis of data, collaborating with Biostatistics and Bioinformatics Research Shared Resources to insure quality of the results, interpreting results and planning new experiments. - (Essential)
  • 30% - Collaborates in preparing manuscripts and grants based on the experimental data generated. Duties include keeping current with the literature, preparing figures based on data collected, writing manuscripts and sections of grants with the PI and senior team members, presenting current work in research in progress seminars, attending departmental seminars and participating in journal clubs. - (Essential)
  • 10% - Helps with laboratory maintenance and regulatory compliance, eg ordering, cleanup of lab areas, maintenance of equipment, helping to prepare IACUC protocols and amendments when appropriate. Ordering of laboratory supplies, maintaining stocks of experimental reagents and solutions, maintaining equipment in good repair, glassware washing and general lab cleanliness. - (Essential)


Preferred Experience & Additional Skills: Strong organizational, record keeping, interpersonal and communication skills required. Must be able to perform complex calculations, to follow complicated experimental procedures and methods and develop independence with tasks and experiments. Preference will be given to candidates with experience with animal husbandry, and colony management. Prior experience in PCR and recombinant DNA work, RNA and protein analysis are required. Some weekend, holidays and/or evenings may be required. Experience in work with mouse genetic models is desirable but not necessary.

Minimum Experience and Training Requirements: The candidate should be highly motivated with a recent Ph.D. degree and a solid background and skills in molecular/cell biology and biochemistry.

Physical Requirements: Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32? F to 100? F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/20 vision, corrected. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous)

The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.

Opening date: 06 Dec 2021 Eastern Standard Time
Applications close:
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