Postdoctoral Fellow

Postdoctoral Fellow

Job ID: req2967
Employee Type: exempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

PROGRAM DESCRIPTION

The Laboratory of Human Retrovirology and Immunoinformatics (LHRI) is composed of three sections: the Basic, the translational, and the Bioinformatics research sections, and has collaborated with the National Institute of Allergy and Infectious Diseases (NIAID). LHRI has supported NIAID clinical studies for patients infected with HIV, HCV, SARS-Cov2, and other viruses. During studies, LHRI has discovered Interleukin-27 (IL-27) as an anti-viral protein, Ku70: a DNA repair protein, as a Type-III Interferon inducing cytosolic DNA receptor, and novel IL-27-inducing anti-viral micro RNAs (miRNAs). LHRI carried over the Database for Annotation, Visualization and Integrated Discovery (DAVID) (https://david.ncifcrf.gov/) from LIB and has maintained/ improved it to support users. The Basic research section is looking for a young, passionate scientist who will dedicate this challenging project.

KEY ROLES/RESPONSIBILITIES

• Investigation of the molecular mechanism of IL-27 and anti-viral miRNA-mediated anti-virus effect, ROS and autophagy induction in multiple cell types.
• Run multiple instrument: confocal microscope (Zeiss), fluorescence microscopy (Zeiss, Nikon), AMINIS;FlowSight imaging flow cytometry, Sanger DNA sequence, Nexseq2000, single-cell RNA-seq, Singel-cell proteomics, Quanterix ELISA system, RNA scope.
• Analysis of Big Data using piplines with staff in the bioinformatic section.
• Publishing paper(s) and presenting the findings in the international or domestic scientific conference(s) as the first author.

**THIS POSITION IS CONTINGENT UPON FUNDING APPROVAL**

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

• Possession of a Ph.D. or M.D./Ph.D. from an accredited college or university according to the Council for Higher Education Accreditation (CHEA) . (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
• No Experience in virus study required with the degree.
• Publication of the first author paper(s).
• Ability to obtain and maintain a security clearance.

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:

• Handling of RNA and RT-PCR
• Construction of plasmid using the in-fusion cloning technology
• Tissue culture (primary cells and cell lines) for long period.
• Transfection of DNA, siRNA or shRNA and Handling of lentiviruses
• Western blotting and ELISA
• Statistical analysis skills

JOB HAZARDS

• This position is subject to working with potential for exposing to infectious materials, requiring medical clearance and immunizations under SOP.
• This position is subject to working with or have potential for exposure to infectious
  material, requiring medical clearance and immunizations.

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)

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