GENERAL PURPOSE :
Under general direction and with considerable latitude for exercise of independent judgment, provides direct research and research training support at the professional level by directing and executing small, complex research projects or major aspects of larger research projects; and provides extensive guidance to and/or directly supervises technicians, undergraduate students, postdoctoral appointees and professional staff.
SPECIFIC RESPONSIBILITIES : Under general direction and with latitude for independent judgment:
Directs a variety of small, complex research projects related to the culture of both primary neural cells and pluripotent stem cells.
Develops analytical protocols for running the BD FACSAria IIIU and BD FACSAria Fusion Cell Sorters. Independently prepares both primary neural cells and the derivatives of pluripotent stem cell lines for fluorescence-activated single cell sorting (FACS) and magnetic-activated cell separation (MACS) analysis, as well as sorting, to meet the specific needs of lab research projects. Develops and modifies test methods; selects procedures for data collection and handling; develops or utilizes computer programs for conversions and statistical treatment of data. Designs research projects or sub-projects, contributes to writing of research proposals, prepares regulatory documentation required for performance of research. Writes protocols for the conduct of research, which include: purposes of study, design of experiments, lists of materials and methods to be used and the schedule of activities.
Analyzes and evaluates microscopic analysis and quantitative procedures for the assessment of cultured cells, and interprets results within the scope of the study; assesses the importance of findings in relation to the general research program. Prepares written reports on all phases of laboratory work involved in research projects. Contributes to the design, interpretation and final writing of research publications - thereby meeting expectations for co-authorship of scientific publications.
Directs initial training as well as continuous skill development of staff/trainees in cell culture techniques and instrumentation procedures. Counsels staff/trainees on technical problems; advises and assists staff/trainees with special research projects.
Keeps abreast of trends in field of interest by reading current research literature, abstracting scientific articles of value in the prosecution of research problems, and attending and presenting at scientific meetings, seminars and research conferences. Develops and controls research project budgets in consultation with supervising faculty. Is responsible for research project budget reports and records. Recommends expenditures for major laboratory equipment and construction. Authorizes expenditure for expendable supplies and maintenance.
Directs the gathering of research materials, supplies, equipment and apparatus and experimental. Coordinates service support: repair of flow cytometer, cell sorters and other major equipment, and, requisitioning of supplies and research materials.
Evaluate the performance of research equipment, making design changes or developing new approaches for its use.
Other projects and assignments as needed.
· Doctoral degree with major course work in the field of assignment and a minimum of 2-5 years of relevant postdoctoral training.
· A good understanding of cell and molecular biology and hands-on experience in mammalian cell culture and with experience in DNA cloning and expression is required. Experience on primary neural cell culture is a plus.
· Working knowledge of FACS highly advantageous.
· Strong interpersonal, presentation and communication skills, including written communication.
· Strong attention to detail, analytical and leadership ability.
EOE Minorities/Females/Protected Veterans/Disabled