Sr. Research Scientist II, Protein Biochemistry

GILEAD Sciences
San Mateo County, CA
Closing date
Dec 4, 2022

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Science, Chemistry, Biochemistry
Organization Type
For Current Gilead Employees and Contractors:
Please log onto your Internal Career Site to apply for this job.
Job Description
The Protein Therapeutics Department in Gilead is looking for a Senior Research Scientist II to help support and expand our protein sciences capabilities. The candidate will join the Protein biochemistry group delivering high-quality recombinant proteins to fulfill the needs of teams focused on creating new therapies in Immunology, Oncology, and Virology.

The ideal candidate has demonstrated expertise in leading multiple protein generation projects to enable small-molecule and large-molecule campaigns. On the technical side, he/she will design constructs required to address the protein needs for biochemical and biophysical assays, including high throughput screening and structure determination via crystallography and Cryo-EM. Proven experience with small-scale expression testing and optimization in multiple hosts ( E. coli , insect, and mammalian cells) for intracellular and secreted proteins is a must. The candidate will use milligram scale expression, purification process development, optimization, and solid protein analytics skills to deliver fit-for-purpose proteins. Experience with producing challenging proteins is desired (highly disordered, low expression yield, aggregation-prone, membrane-bound proteins).

Additional responsibilities for this role include managing, training, and mentoring junior and senior scientists to fulfill project needs by delivering high-quality proteins on time.

The candidate will interact closely and collaborate across all functions of Drug Discovery -Biology, Biologics Discovery, Structural and Computational Biology, and Biophysical and Biochemical Assay within Gilead. In addition, the scientist will be expected to help build protein generation capacity and capabilities by managing and establishing collaborations with CROs and academic laboratories.

The position will require the scientist to work creatively, collaboratively, and in a fast-paced environment. He/she will also be encouraged to test and implement new technologies that further enhance the capabilities of the Protein Biochemistry team or address specific challenges in protein generation.

Education and Technical Skills:
  • Ph.D. with 5+ years of Biochemistry research experience, including 5+ years in Protein Sciences in an industry setting.
  • A proven track record of independently producing quality proteins from several expression hosts (mammalian, insect, and bacterial cells) is required.
  • Training in classical protein production processes and techniques (Molecular Biology, small-scale expression, and purification, affinity capture, ion exchange, size exclusion chromatography using AKTA chromatographic instruments, large-scale protein purification process development and optimization (milligrams))
  • Expert knowledge of protein sciences analytics (sequence alignment, protein structure analysis using modeling software, SDS PAGE analysis, Western blot, FPLC and HPLC, DLS, DSF, Mass Spectrometry)
  • Experience building and managing a protein sciences team and laboratory is strongly desired.
  • Ability to establish and foster productive collaborations with internal and external partners, including academic labs and CROs, is required.
  • Excellent communication and presentation skills; works independently and as a team player.
  • Attention to detail, accurate record keeping, and good lab citizenship practices are essential.
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For Current Gilead Employees and Contractors:
Please log onto your Internal Career Site to apply for this job.
  • Location: United States - California - Foster City
  • Type: Full time

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