Multiomics Research Professional

Stanford University is seeking a Life Science Research Professional 3 (LSRP3) to provide highly multiplexed spatial proteomics services (CODEX) in the School of Medicine, Beckman Center's Cell Sciences Imaging Facility (CSIF). The CSIF provides high-resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells and tissue as well as bioengineered materials to all Stanford University and industry researchers. The facility offers sophisticated and demanding microscopy techniques (e.g. super-resolution, confocal, FLIM, FRET, FRAP, 2-photon imaging, photo-activation and uncaging, spatial proteomics as well as image processing and analysis.)

LSRP3 will develop and apply expert knowledge in both the theory and application of microscopy as it applies to highly multiplexed spatial proteomics. LSRP3 will also possess a wide-ranging knowledge of molecular, cellular, and whole-organism anatomy and function, particularly those related to the common organisms and diseases (e.g. cancers, neurodegenerative diseases, etc.) studied by Stanford's research community. This position desires experience in clinical sample preparation, fluorescence-based immunohistochemistry (IHC) labeling of tissue culture and sample slices (frozen and FFPE), extensive imaging of subcellular and tissue level structures, and interpreting multiparametric microscopy data. The position further requires expert-level experience using image analysis and processing software (e.g. ImageJ/FIJI, Volocity, Imaris, and/or Huygens). Experience in visualizing and analyzing high dimensional data with VisioPharm software is a plus. Must possess demonstrations of excellent written and verbal communication skills (publications and presentation in related research field), organizational and multi-tasking ability in lab managing, as well as excellent interpersonal and customer service skills. Must be able to work with and meet project deadlines.

Duties include:

• Execute CODEX imaging portions of research project(s), including background research, experimental design and execution, problem solving, and writing up for publication.
• Develop new protocols; test and evaluate a variety of approaches particularly in the development and application of high parameter multiplexing and multiparametric imaging methods of tissue slices using a CODEX imaging platform.
• Assist in development and validation of new antibody panels for application on the CODEX imaging platform.
• Conduct imaging experiments and maintain detailed records of experiments and outcomes; perform comprehensive data analysis on VisioPharm software, analyze and interpret research data resulting from original procedures and methods.
• Will advise, design, and help execute imaging experiments of both fixed and frozen samples, with students, researchers.
• Prepare periodic progress reports or written reports on research experiments and projects to CSIF director.
• Participate in research teams across different faculties or schools.
• Contribute to the preparation of manuscripts for publication, especially in the methods and results section. May be a co-author on publications depending on the level of intellectual contribution. Present ongoing work and findings to colleagues at academic conferences.
• Perform ongoing literature review to remain current with new procedures and related research; propose new research directions based on new advances discussed/proposed in literature.
• Oversee supply and equipment budget under minimal supervision.
• Work with facility manager to ensure lab complies with regulations and all required trainings.
• Train new staff as needed. Provide instructions on techniques and consultation on project work.
• Conduct monthly facility billing routines from iLabs.

* Other duties may also be assigned

DESIRED QUALIFICATIONS:

• Hands-on experience in multiplexed immunofluorescent (IF) staining of FFPE and fresh frozen (FF) tissue sections.
• Extensive and established multiplexed IF data acquisition, interpretation, and presenting skills.
• Direct experience in and comprehensive understanding of spatial-omics and/or transcriptomics studies (whole-genome library preparation experience is a plus).
• Ability to adapt to different working environments and to work with diverse collaborators is favored.

EDUCATION & EXPERIENCE (REQUIRED):

Bachelor's degree in related scientific field and four years of relevant work experience; or Master's degree in a related scientific field and two years relevant experience; or PhD in related science field.

KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):

• Comprehensive understanding of scientific principles.
• Expert level knowledge and skills in multiplexed IF staining and imaging using clinical tissue sections (FFPE and FF).
• General computer skills, experience with data/image analysis and scientific applications, and ability to quickly learn and master computer programs.
• Strong analytical skills and excellent judgment.
• Ability to multi-task and meet deadlines with general guidance is essential.
• Excellent organizational skills and demonstrated ability to complete detailed work accurately.
• Developing project management skills.

CERTIFICATIONS & LICENSES:

None

PHYSICAL REQUIREMENTS*:

• Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
• Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
• Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
• Must have correctible vision to perform duties of the job.
• Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
• Ability to lift heavy objects weighing up to 50 pounds.
• Ability to work in a dusty, dirty, and odorous environment.
• Position may require repetitive motion.

* - Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.

WORKING CONDITIONS:

• May require working in close proximity to blood borne pathogens.
• May require work in an environment where animals are used for teaching and research.
• Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
• Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
• May require extended or unusual work hours based on research requirements and business needs.

WORK STANDARDS:

• Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
• Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
• Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu.

The expected pay range for this position is $82,000 to $102,000 per annum. Stanford University provides pay ranges representing its good faith estimate of what the university reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location and external market pay for comparable jobs.

As an organization that receives federal funding, Stanford University has a COVID-19 vaccination requirement that will apply to all university employees. To learn more about COVID policies and guidelines for Stanford University Staff, please visit https://cardinalatwork.stanofrd.edu/working-stanford/covid-19-guide/interim-policies/covid-19-vaccination-and-surveillance-testing

Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law.