Research Associate - Marson lab

Gladstone Institutes
San Bruno, CA
Closing date
Oct 6, 2023
Lab/Area:Marson Lab


The Gladstone-UCSF Institute of Genomic Immunology is recruiting a Research Associate (RA) to help perform in vivo experiments in mouse models of immunotherapies and assist with the management of the mouse colony used for ongoing multidisciplinary cellular engineering projects. Our institute has pioneered the application of novel genetic engineering technologies such as CRISPR to primary human immune cells. These new technical abilities have furthered our research into the genetic control of T cell biology as well as opened the door to novel cancer immunotherapies based on engineered human immune cells.

Key responsibilities

The RA will assist with the management and maintenance of institute mouse colonies located at Gladstone and UCSF. They will be trained in experimental procedures involving mouse models of cancer and autoimmune disease and participate in rigorous assessments of the in vivo efficacy of T cell therapies in these models. Tasks require routine handling of mice.
  • Perform routine mouse colony management duties (ear punching/ear tagging/tailing for genotyping, weaning, aging and sexing of pups, and euthanasia, et al)
  • Identify and implement required care for mice with health issues and mice on special treatment (special diet, fasting, treated water).
  • Maintain mouse database using Google sheets or mouse colony software.
  • Primer design and PCR troubleshooting
  • Perform PCR followed by electrophoresis or Sanger sequencing for genotyping
  • Place genotyping orders in TransnetYX and interpret the genotyping results
  • Collect blood samples via submandibular bleeding for downstream analysis
  • Learn and become proficient with techniques related to cancer models, such as intravenous injections of cancer cell lines and/or immune cells by tail vein injection or retro-orbital injection
  • Administer medication/reagents orally and via subQ, IP, IM, or IV
  • Assist with in vivo imaging in cancer models (bioluminescence)
  • Assist with cell culture and preparation for subQ injections or IV injections
  • Assist with dissection of spleen, lymph nodes, engrafted tumor for downstream analysis
  • Work collaboratively across a dynamic scientific environment
  • Maintain and ensure safe laboratory environment and work practices. Follow standard operating procedures (SOP) and IACUC protocols.
  • Other related duties as assigned

Required Qualifications:
  • BA/BS degree and one or more years of laboratory experience utilizing techniques or methods required by the position; or an equivalent combination of education and experience
  • New graduates with a commitment to learning are welcome to apply
  • Must be able to work with mouse colonies
  • Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.)
  • Excellent attention to detail, organizational and interpersonal communication skills (verbal and written)
  • Willingness and ability to learn new methods and skills for changing research priorities
  • Ability to work independently and as a member of a research team
  • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines
  • Ability to comply with policies and procedures
  • Some evening and weekend hours are required

Preferred Qualifications
  • Prior experience with mammalian cell culture, especially T cell culture
  • Prior experience with DNA sequencing technologies, including Sanger and NGS

The Gladstone-UCSF Institute of Genomic Immunology is committed to improving diversity, equity, and inclusion in the genomic immunology field, from trainees to faculty, and is an equal opportunity employer.

Hiring Range:


Additional Information:
  • Title and salary will be commensurate with education and experience.
  • Gladstone is an equal opportunity employer.

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